風(fēng)麻疹病毒二聯(lián)檢診斷試紙（酶聯(lián)免疫法）

廣州健侖生物科技有限公司

廣州健侖長(zhǎng)期供應(yīng)各種ELISA試劑盒，主要代理進(jìn)口和國(guó)產(chǎn)品牌的流行病毒ELISA檢測(cè)試劑盒。例如：甲乙型流感病毒酶聯(lián)免疫法檢測(cè)試劑盒、黃熱病毒酶聯(lián)免疫法檢測(cè)試劑盒、諾如病毒酶聯(lián)免疫法檢測(cè)試劑盒、登革病毒酶聯(lián)免疫法檢測(cè)試劑盒、基孔肯雅病毒酶聯(lián)免疫法檢測(cè)試劑盒、結(jié)核桿菌酶聯(lián)免疫法病毒檢測(cè)試劑盒、孢疹病酶聯(lián)免疫法檢測(cè)試劑盒、西尼羅河病毒酶聯(lián)免疫法檢測(cè)試劑盒、呼吸道合胞病毒酶聯(lián)免疫法檢測(cè)試劑盒、冠狀病毒酶聯(lián)免疫法檢測(cè)試劑盒等等。蟲媒體染病系列、呼吸道病原體系列、發(fā)熱伴出疹系列、消化道及食源感染系列。

檢驗(yàn)原理風(fēng)麻疹病毒二聯(lián)檢診斷試紙（酶聯(lián)免疫法）

用抗原包被微量板孔，制成固相載體。加患者血清到板孔中，其所含的抗體特異性地與固相載體中現(xiàn)存抗原結(jié)合，形成免疫復(fù)合物。除去多余物質(zhì)后，加入結(jié)合了堿性磷酸酶的IgG、IgA或IgM抗體，使之與上述免疫復(fù)合物反應(yīng)。洗板，除去多余的結(jié)合物，加入底物（對(duì)硝基苯磷酸鹽）。其與酶結(jié)合的免疫復(fù)合物反應(yīng)，產(chǎn)生有顏色產(chǎn)物，顏色強(qiáng)度與特異性抗體含量成正比。

產(chǎn)品規(guī)格：96T/盒

存儲(chǔ)條件：4-8℃

ELISA法：用滅活風(fēng)疹病毒抗原包被的載體可與被檢樣品中的特異性抗體結(jié)合，用酶標(biāo)記的抗人免疫球蛋白與底物檢測(cè)相應(yīng)抗體。
試劑
除滅活風(fēng)疹病毒抗原包被的微孔板，陰、陽(yáng)性參考血清外，其余同一般ELISA。
操作方法
（1）樣品采集，作過(guò)篩試驗(yàn)僅需采集單份血樣，但若需判斷病毒感染者的免疫狀態(tài)，需對(duì)可疑風(fēng)疹患者在出現(xiàn)斑疹后3天內(nèi)以及隨后的14～21天分別取樣同時(shí)檢測(cè)。
（2）同一般ELISA，在對(duì)照和樣品各孔中加PBS 50μl，繼加樣品10μl，25℃保溫45min，洗滌、吸干。
（3）各孔加酶標(biāo)記物250μl，同法保溫和洗滌。
（4）再加pNPP底物溶液250μl，同法保溫和洗滌后，加1mol/L氫氧化鈉50μl中止反應(yīng)，測(cè)定各孔吸光度值405nm，判斷被測(cè)樣品結(jié)果。
（5）若為陽(yáng)性結(jié)果，可將樣品作進(jìn)一步稀釋后作抗體滴度測(cè)定，比較先后兩份樣品結(jié)果，并進(jìn)行判斷（表1）：

判定
1.風(fēng)疹病毒的IgG和lgM抗體均為陽(yáng)性，或者是IgG抗體滴度≥1：512，表明有風(fēng)疹病毒近期感染。
2.風(fēng)疹病毒的IgG和IgM抗體均為陰性，表明沒(méi)有受過(guò)風(fēng)疹病毒感染。
3.風(fēng)疹病毒的lgG抗體滴度<1：512，lgM抗體為陰性，說(shuō)明有過(guò)既往感染史。
4.此外，再次感染風(fēng)疹病毒，由于只出現(xiàn)短暫的IgM抗體或出現(xiàn)的水平很低，所以不易查出。因此風(fēng)疹病毒IgG抗體滴度在雙份血清中有4倍以上升高，那么無(wú)論lgM抗體是否為陽(yáng)性，都是風(fēng)疹病毒近期感染的指標(biāo)。

ELISA method: The carrier coated with inactivated rubella virus antigen can be combined with the specific antibody in the sample to be tested, and the corresponding antibody is detected with enzyme-labeled anti-human immunoglobulin and substrate.
Reagents
In addition to the inactivated rubella virus antigen coated microplate, negative and positive reference serum, the rest of the same ELISA.
Method of operation
(1) sample collection, sieve test only need to collect a single blood sample, but if you need to determine the immune status of the virus-infected persons, the need for suspected rubella patients within 3 days after the emergence of rash and subsequent sampling of 14 to 21 days at the same time Detection.
(2) In the same ELISA, add PBS 50μl to each well of control and sample, add 10μl of sample, incubate at 25 ℃ for 45min, wash and dry.
(3) Each well plus enzyme labeling 250μl, with the law insulation and washing.
(4) add pNPP substrate solution 250μl, the same method of incubation and washing, add 1mol / L sodium hydroxide 50μl reaction was stopped, the determination of the absorbance of each well 405nm value, determine the test sample results.
(5) If the result is positive, the sample can be further diluted for antibody titers. The results of the two samples are compared and judged (Table 1):

determination
1. Rubella IgG and lgM antibodies are positive, or IgG antibody titers ≥ 1: 512, indicating a recent infection with rubella virus.
Rubella virus IgG and IgM antibodies were negative, indicating that they have not been rubella virus infection.
3. Rubella virus lgG antibody titer <1: 512, lgM antibody negative, indicating that there was a history of previous infections.
4. In addition, re-infection with rubella virus, due to the emergence of transient IgM antibodies or the emergence of low levels, so difficult to detect. Therefore, rubella virus IgG antibody titers in double serum increased more than four times, then whether lgM antibodies are positive, are indicators of recent infection with rubella virus.

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103

病毒存在于出疹前5～7天病兒唾液及血液中，但出疹2天后就不易找到。風(fēng)疹病毒在體外生活力很弱，但傳染性與麻疹一樣強(qiáng)。好發(fā)于5歲以下的嬰幼兒，6個(gè)月以內(nèi)嬰兒因有來(lái)自母體的抗體獲得抵抗力，很少發(fā)病。一次得病，可終身免疫，很少再次患病。春夏之交，風(fēng)疹病毒也在欲動(dòng)，它會(huì)伴隨人的咳嗽和噴嚏而漂浮在空氣中。抵抗力較弱的人吸入風(fēng)疹病毒后，經(jīng)過(guò)2~3周的潛伏期，便開始出現(xiàn)癥狀。先是全身不適，繼而出現(xiàn)發(fā)熱、耳后及枕部淋巴結(jié)腫大，并有淡紅色細(xì)點(diǎn)狀丘疹，短期內(nèi)擴(kuò)展到全身，奇癢難耐或微癢，多在2~3天內(nèi)消退，*跡。由于風(fēng)疹的癥狀和體征與感冒及蕁麻疹相似，因而不太引起人們的重視。
風(fēng)疹從接觸感染到癥狀出現(xiàn)，要經(jīng)過(guò)14～21天。病初1～2天癥狀很輕，可有低熱或中度發(fā)熱，輕微咳嗽、乏力、胃口不好、咽痛和眼發(fā)紅等輕度上呼吸道癥狀。病人口腔粘膜光滑，無(wú)充血及粘膜斑，耳后、枕部淋巴結(jié)腫大，伴輕度壓痛。通常于發(fā)熱1～2天后出現(xiàn)皮疹，皮疹先從面頸部開始，在24小時(shí)蔓延到全身。皮疹初為稀疏的紅色斑丘疹，以后面部及四肢皮疹可以融合，類似麻疹。出疹第二天開始，面部及四肢皮疹可變成針尖樣紅點(diǎn)，如猩紅熱樣皮疹。
皮疹一般在3天內(nèi)迅速消退，留下較淺色素沉著。在出疹期體溫不再上升，病兒常無(wú)疾病感覺(jué)，飲食嬉戲如常。風(fēng)疹與麻疹不同，風(fēng)疹全身癥狀輕，無(wú)麻疹粘膜斑，伴有耳后、頸部淋巴結(jié)腫大。
風(fēng)疹是病毒性傳染病，臨床特點(diǎn)是：全身癥狀輕微，皮膚紅色斑丘疹及枕后、耳后、頸后淋巴結(jié)腫大伴觸痛，合并癥少見。病毒可通過(guò)胎盤傳給胎兒而致各種先天缺陷，稱為先天性風(fēng)疹綜合征。

The virus is present in the saliva and blood of sick children 5 to 7 days before the rash but is not easy to find after 2 days of rash. Rubella virus has a weak viability in vitro but is as contagious as measles. Occur in infants and children under 5 years of age, within 6 months of infants derived from the mother's antibody resistance, rarely disease. Once sick, lifelong immunity, rarely again sick. At the turn of spring and summer, the rubella virus is just around the corner, floating in the air with coughing and sneezing. People with weaker immunity inhaled rubella virus, after 2 to 3 weeks of incubation period, began to show symptoms. First, general malaise, followed by fever, ear and occipital lymph nodes, and fine pink papules, extended to the body within a short period of time, itchy emboldened or slightly itchy, mostly in 2 to 3 days subsided, leaving no trace. Because the symptoms and signs of rubella are similar to those of flu and urticaria, they are less of a cause for concern.
Rubella from infection to symptoms, to go through 14 to 21 days. 1 to 2 days early disease symptoms are very mild, may have low or moderate fever, mild cough, fatigue, poor appetite, sore throat and eye redness mild upper respiratory symptoms. Patients with smooth oral mucosa, no congestion and mucosal plaque, ear, occipital lymph nodes, with mild tenderness. Usually 1 to 2 days after the fever rash, rash first from the face and neck, spread to the body in 24 hours. Rash at early sparse red rash, after facial and limb rash can be integrated, similar to measles. Rash the next day, the face and limb rash can become a needle-like red dot, such as scarlet fever-like rash.
The rash subsided rapidly within 3 days, leaving a more pale pigmentation. In the rash of the body temperature is no longer rising, sick children often have no sense of disease, diet playful as usual. Rubella and measles are different, rubella systemic symptoms of light, no measles mucosa, accompanied by ear, neck lymph nodes.
Rubella is a viral infectious disease, clinical features are: minor symptoms, red rash and occiput skin, behind the ear, neck and neck lymph nodes with tenderness, complications rare. The virus can be passed to the fetus through the placenta caused by a variety of birth defects, known as congenital rubella syndrome.